Chronic hyperinsulinemia promotes human hepatocyte senescence.

ABSTRACT:

Objective

Cellular senescence, an irreversible proliferative cell arrest, is caused by excessive intracellular or extracellular stress/damage. Increased senescent cells have been identified in multiple tissues in different metabolic and other aging-related diseases. Recently, several human and mouse studies emphasized the involvement of senescence in development and progression of NAFLD. Hyperinsulinemia, seen in obesity, metabolic syndrome, and other conditions of insulin resistance, has been linked to senescence in adipocytes and neurons. Here, we investigate the possible direct role of chronic hyperinsulinemia in the development of senescence in human hepatocytes.

Methods

Using fluorescence microscopy, immunoblotting, and gene expression, we tested senescence markers in h

SUBMITTER: Baboota RK 

PROVIDER: S-EPMC9364104 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature