Identification of heterogeneous subsets of aortic interleukin-17A-expressing CD4⁺ T cells in atherosclerotic mice.

ABSTRACT:

Objectives

T helper 17 (Th17) cells are involved in the inflammatory response of atherosclerosis. However, their heterogeneity in the atherosclerotic aorta remains elusive. This study was designed to identify aortic Th17 subsets.

Methods

The surface markers and transcription factors of aortic interleukin-17A (IL-17A)-expressing T cells were determined by flow cytometry in an ApoE-deficient mouse atherosclerotic model. Viable aortic IL-17A-expressing T cell subsets were isolated by flow cytometry on the basis of surface markers, followed by characterizing their transcription factors by either flow cytometry or real-time RT-PCR. The effect of aortic IL-17A-expressing T cell subsets on aortic endothelial cells was determined in vitro.

Results

C-X-C Motif Chemokine Recept

SUBMITTER: Lin G 

PROVIDER: S-EPMC9364180 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature