Human Vimentin Layers on Solid Substrates: Adsorption Kinetics and Corona Formation Investigations.

ABSTRACT: Adsorption kinetics of human vimentin on negatively charged substrates (mica, silica, and polymer particles) was analyzed using atomic force microscopy (AFM), quartz microbalance (QCM), and the laser doppler velocimetry (LDV) method. AFM studies realized under diffusion conditions proved that the adsorbed protein layer mainly consisted of aggregates in the form of compact tetramers and hexamers of a size equal to 11-12 nm. These results were consistent with vimentin adsorption kinetics under flow conditions investigated by QCM. It was established that vimentin aggregates efficiently adsorbed on the negatively charged silica sensor at pH 3.5 and 7.4, forming compact layers with the coverage reaching 3.5 mg m^-2. Additionally, the formation of the vimentin corona at polymer particl

SUBMITTER: Wasilewska M 

PROVIDER: S-EPMC9364323 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature