Kinesin-3 motors are fine-tuned at the molecular level to endow distinct mechanical outputs.

ABSTRACT:

Background

Kinesin-3 family motors drive diverse cellular processes and have significant clinical importance. The ATPase cycle is integral to the processive motility of kinesin motors to drive long-distance intracellular transport. Our previous work has demonstrated that kinesin-3 motors are fast and superprocessive with high microtubule affinity. However, chemomechanics of these motors remain poorly understood.

Results

We purified kinesin-3 motors using the Sf9-baculovirus expression system and demonstrated that their motility properties are on par with the motors expressed in mammalian cells. Using biochemical analysis, we show for the first time that kinesin-3 motors exhibited high ATP turnover rates, which is 1.3- to threefold higher compared to the well-studied kinesin-

SUBMITTER: Soppina P 

PROVIDER: S-EPMC9364601 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature