Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing.

ABSTRACT:

Background

Studies in vertebrate genomics require sampling from a broad range of tissue types, taxa, and localities. Recent advancements in long-read and long-range genome sequencing have made it possible to produce high-quality chromosome-level genome assemblies for almost any organism. However, adequate tissue preservation for the requisite ultra-high molecular weight DNA (uHMW DNA) remains a major challenge. Here we present a comparative study of preservation methods for field and laboratory tissue sampling, across vertebrate classes and different tissue types.

Results

We find that storage temperature was the strongest predictor of uHMW fragment lengths. While immediate flash-freezing remains the sample preservation gold standard, samples preserved in 95% EtOH or 20-25% D

SUBMITTER: Dahn HA 

PROVIDER: S-EPMC9364683 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature