Modular capsid decoration boosts adenovirus vaccine-induced humoral immunity against SARS-CoV-2.

ABSTRACT: Adenovirus vector vaccines have been widely and successfully deployed in response to coronavirus disease 2019 (COVID-19). However, despite inducing potent T cell immunity, improvement of vaccine-specific antibody responses upon homologous boosting is modest compared with other technologies. Here, we describe a system enabling modular decoration of adenovirus capsid surfaces with antigens and demonstrate potent induction of humoral immunity against these displayed antigens. Ligand attachment via a covalent bond was achieved using a protein superglue, DogTag/DogCatcher (similar to SpyTag/SpyCatcher), in a rapid and spontaneous reaction requiring only co-incubation of ligand and vector components. DogTag was inserted into surface-exposed loops in the adenovirus hexon protein to allow attachme

SUBMITTER: Dicks MDJ 

PROVIDER: S-EPMC9364715 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature