Variability in cell-free expression reactions can impact qualitative genetic circuit characterization.

ABSTRACT: Cell-free expression systems provide a suite of tools that are used in applications from sensing to biomanufacturing. One of these applications is genetic circuit prototyping, where the lack of cloning is required and a high degree of control over reaction components and conditions enables rapid testing of design candidates. Many studies have shown utility in the approach for characterizing genetic regulation elements, simple genetic circuit motifs, protein variants or metabolic pathways. However, variability in cell-free expression systems is a known challenge, whether between individuals, laboratories, instruments, or batches of materials. While the issue of variability has begun to be quantified and explored, little effort has been put into understanding the implications of this variabi

SUBMITTER: Rhea KA 

PROVIDER: S-EPMC9365049 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature