BlotIt-Optimal alignment of Western blot and qPCR experiments.

ABSTRACT: Biological systems are frequently analyzed by means of mechanistic mathematical models. In order to infer model parameters and provide a useful model that can be employed for systems understanding and hypothesis testing, the model is often calibrated on quantitative, time-resolved data. To do so, it is typically important to compare experimental measurements over broad time ranges and various experimental conditions, e.g. perturbations of the biological system. However, most of the established experimental techniques such as Western blot, or quantitative real-time polymerase chain reaction only provide measurements on a relative scale, since different sample volumes, experimental adjustments or varying development times of a gel lead to systematic shifts in the data. In turn, the number of

SUBMITTER: Kemmer S 

PROVIDER: S-EPMC9365137 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature