Single-cell multiomics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection.

ABSTRACT: Round spermatid injection (ROSI) technique holds great promise for clinical treatment of a proportion of infertile men. However, the compromised developmental potential of ROSI embryos largely limits the clinical application, and the mechanisms are not fully understood. Here, we describe the transcriptome, chromatin accessibility, and DNA methylation landscapes of mouse ROSI embryos derived from early-stage round spermatids using a single-cell multiomics sequencing approach. By interrogating these data, we identify the reprogramming defects in ROSI embryos at the pronuclear stages, which are mainly associated with the misexpression of a cohort of minor zygotic genome activation genes. We screen a small compound, A366, that can significantly increase the developmental potential of ROSI embr

SUBMITTER: Wang J 

PROVIDER: S-EPMC9365279 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature