First case of SARS-CoV-2 RNA detection in municipal solid waste leachate from Brazil.

ABSTRACT: This work presents the first case of SARS-CoV-2 RNA detection in leachate collected from a transfer station in the city of São Paulo, Brazil. After calibration of the viral detection method already used for wastewater samples with a pilot leachate sample and virus fragments in laboratory, twelve polyethylene glycol concentrated leachates samples were tested by RT-qPCR. The results confirmed the presence of N1 gene in 9 of the 12 analyzed samples between epidemiological weeks 33 and 38 of the year 2021 (08/15/2021 to 09/19/2021). The occurrence of the N2 gene was only observed in 5 of the 12 samples. The concentration values for N1 and N2 genes varied between 3.1 and 4.6 log₁₀.GC·L^-1, which are values close to those measured in sanitary wastewater. This method showed t

SUBMITTER: Mondelli G 

PROVIDER: S-EPMC9365452 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature