Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury.

ABSTRACT:

Background

Acute kidney injury (AKI) is the most common target organ damage in sepsis. Sepsis-associated AKI (SA-AKI) may be characterized by damage to the renal tubular epithelium. In this study, the pharmacological mechanisms of Astragalus membranaceus and its active monomer Astragaloside IV (AS-IV) were predicted based on a network pharmacology approach and validated both in vitro and in vivo using the SA-AKI model.

Method

We constructed an in vivo sepsis model using a mouse cecum ligation puncture (CLP) and HK-2 cells were treated with lipopolysaccharide (LPS) to mimic Gram (-) induced sepsis to assess the renal-protective efficacy of Astragalus membranaceus and AS-IV.

Results

The findings demonstrated that Astragalus membranaceus and AS-IV at

SUBMITTER: Tang JL 

PROVIDER: S-EPMC9365550 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature