Karyotype engineering reveals spatio-temporal control of replication firing and gene contacts.

ABSTRACT: Eukaryotic genomes vary in terms of size, chromosome number, and genetic complexity. Their temporal organization is complex, reflecting coordination between DNA folding and function. Here, we used fused karyotypes of budding yeast to characterize the effects of chromosome length on nuclear architecture. We found that size-matched megachromosomes expand to occupy a larger fraction of the enlarged nucleus. Hi-C maps reveal changes in the three-dimensional structure corresponding to inactivated centromeres and telomeres. De-clustering of inactive centromeres results in their loss of early replication, highlighting a functional correlation between genome organization and replication timing. Repositioning of former telomere-proximal regions on chromosome arms exposed a subset of contacts betwee

SUBMITTER: Lazar-Stefanita L 

PROVIDER: S-EPMC9365758 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature