Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum.

ABSTRACT: Clostridium acetobutylicum is a promising biocatalyst for the renewable production of n-butanol. Several metabolic strategies have already been developed to increase butanol yields, most often based on carbon pathway redirection. However, it has previously demonstrated that the activities of both ferredoxin-NADP⁺ reductase and ferredoxin-NAD⁺ reductase, whose encoding genes remain unknown, are necessary to produce the NADPH and the extra NADH needed for butanol synthesis under solventogenic conditions. Here, we purify, identify and partially characterize the proteins responsible for both activities and demonstrate the involvement of the identified enzymes in butanol synthesis through a reverse genetic approach. We further demonstrate the yield of butanol formation is

SUBMITTER: Foulquier C 

PROVIDER: S-EPMC9365771 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature