Validation of droplet digital PCR for cytokeratin 19 mRNA detection in canine peripheral blood and mammary gland.

ABSTRACT: In humans, peripheral blood cytokeratin 19 (CK19) mRNA-positive circulating tumor cells (CTCs) was utilized to identify early-stage breast cancer patients with micrometastatic disease who are at risk for disease progression and monitor treatment response in patients with advanced disease. To our knowledge, there has been little research regarding CK19 in canine mammary tumors (CMTs) using molecular methods. A droplet digital PCR (ddPCR) is proposed as a precise and sensitive quantification of nucleic acid targets. Hence, this study aimed to validate a newly designed assay for CK19 detection in canine blood and mammary tissue, along with the reference gene HPRT, by ddPCR. All primers and probes showed a precise match with the exon region of target genes. The assay exhibited PCR efficacy of

SUBMITTER: Tanvetthayanont P 

PROVIDER: S-EPMC9365843 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature