Fabrication on the microscale: a two-photon polymerized device for oocyte microinjection.

ABSTRACT:

Purpose

Intracytoplasmic sperm injection (ICSI) addresses male sub-fertility by injecting a spermatozoon into the oocyte. This challenging procedure requires the use of dual micromanipulators, with success influenced by inter-operator expertise. We hypothesized that minimizing oocyte handling during ICSI will simplify the procedure. To address this, we designed and fabricated a micrometer scale device that houses the oocyte and requires only one micromanipulator for microinjection.

Methods

The device consisted of 2 components, each of sub-cubic millimeter volume: a Pod and a Garage. These were fabricated using 2-photon polymerization. Toxicity was evaluated by culturing single-mouse presumptive zygotes (PZs) to the blastocyst stage within a Pod, with several Pods (and embryo

SUBMITTER: Yagoub SH 

PROVIDER: S-EPMC9365896 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature