Mutation analysis of circulating tumor DNA and paired ascites and tumor tissues in ovarian cancer.

ABSTRACT: Circulating tumor DNA (ctDNA) is one conventional type of liquid biopsy that can be collected to dynamically monitor disease status. However, its potential clinical value and concordance with ascites samples or tumor biopsy needs to be evaluated further for patients with ovarian cancer. Therefore, the present study compared the mutation profiles among ctDNA, paired tumor tissue and ascites samples to explore their possible clinical value in ovarian cancer. Targeted next-generation sequencing was used to screen for mutations in 18 peripheral blood samples, six paired ascites samples and eight paired tumor tissues collected from patients with ovarian cancer. Functional analyses were performed using public databases. WebGestalt was used to perform Gene Ontology and pathway enrichment analyses

SUBMITTER: Jie X 

PROVIDER: S-EPMC9366257 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature