Coupling of phagocytic NADPH oxidase activity and mitochondrial superoxide production.

ABSTRACT: Superoxide radical plays an important role in redox cell signaling and physiological processes; however, overproduction of superoxide or insufficient activity of antioxidants leads to oxidative stress and contributes to the development of pathological conditions such as endothelial dysfunction and hypertension. Meanwhile, the studies of superoxide in biological systems represent unique challenges associated with short lifetime of superoxide, insufficient reactivity of the superoxide probes, and lack of site-specific detection of superoxide. In this work we have developed ¹⁵N-and deuterium-enriched spin probe ¹⁵N-CAT1H for high sensitivity and site-specific detection of extracellular superoxide. We have tested simultaneous tracking of extracellular superoxide by ¹

SUBMITTER: Dikalov SI 

PROVIDER: S-EPMC9366351 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature