The dynamic transcriptome during maturation of biofilms formed by methicillin-resistant Staphylococcus aureus.

ABSTRACT:

Background

Methicillin-resistant Staphylococcus aureus (MRSA), a leading cause of chronic infections, forms prolific biofilms which afford an escape route from antibiotic treatment and host immunity. However, MRSA clones are genetically diverse, and mechanisms underlying biofilm formation remain under-studied. Such studies form the basis for developing targeted therapeutics. Here, we studied the temporal changes in the biofilm transcriptome of three pandemic MRSA clones: USA300, HEMRSA-15, and ST239.

Methods

Biofilm formation was assessed using a static model with one representative strain per clone. Total RNA was extracted from biofilm and planktonic cultures after 24, 48, and 72 h of growth, followed by rRNA depletion and sequencing (Illumina Inc., San Diego, CA, Un

SUBMITTER: Vlaeminck J 

PROVIDER: S-EPMC9366926 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature