Ca²⁺ -dependent interactions between lipids and the tumor-targeting peptide pHLIP.

ABSTRACT: Cancerous tissues undergo extensive changes to their cellular environments that differentiate them from healthy tissues. These changes include changes in extracellular pH and Ca²⁺ concentrations, and the exposure of phosphatidylserine (PS) to the extracellular environment, which can modulate the interaction of peptides and proteins with the plasma membrane. Deciphering the molecular mechanisms of such interactions is critical for advancing the knowledge-based design of cancer-targeting molecular tools, such as pH-low insertion peptide (pHLIP). Here, we explore the effects of PS, Ca²⁺ , and peptide protonation state on the interactions of pHLIP with lipid membranes. Cellular studies demonstrate that exposed PS on the plasma membrane promotes pHLIP targeting. The magnit

SUBMITTER: Vasquez-Montes V 

PROVIDER: S-EPMC9366937 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature