The telomere maintenance mechanism spectrum and its dynamics in gliomas.

ABSTRACT:

Background

The activation of the telomere maintenance mechanism (TMM) is one of the critical drivers of cancer cell immortality. In gliomas, TERT expression and TERT promoter mutation are considered to reliably indicate telomerase activation, while ATRX mutation and/or loss indicates an alternative lengthening of telomeres (ALT). However, these relationships have not been extensively validated in tumor tissues.

Methods

Telomerase repeated amplification protocol (TRAP) and C-circle assays were used to profile and characterize the TMM cross-sectionally (n = 412) and temporally (n = 133) across glioma samples. WES, RNA-seq, and NanoString analyses were performed to identify and validate the genetic characteristics of the TMM groups.

Results

We show through the direct mea

SUBMITTER: Kim S 

PROVIDER: S-EPMC9367055 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature