From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris.

ABSTRACT:

Background

Expansion of the genetic code is a frequently employed approach for the modification of recombinant protein properties. It involves reassignment of a codon to another, e.g., unnatural, amino acid and requires the action of a pair of orthogonal tRNA and aminoacyl tRNA synthetase modified to recognize only the desired amino acid. This approach was applied for the production of trastuzumab IgG carrying p-azido-L-phenylalanine (pAzF) in the industrial yeast Pichia pastoris. Combining the knowledge of protein folding and secretion with bioreactor cultivations, the aim of the work was to make the production of monoclonal antibodies with an expanded genetic code cost-effective on a laboratory scale.

Results

Co-translational transport of proteins into the endoplasmic reti

SUBMITTER: Tir N 

PROVIDER: S-EPMC9367057 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature