The Differential Metabolic Signature of Breast Cancer Cellular Response to Olaparib Treatment.

ABSTRACT: Metabolic reprogramming and genomic instability are key hallmarks of cancer, the combined analysis of which has gained recent popularity. Given the emerging evidence indicating the role of oncometabolites in DNA damage repair and its routine use in breast cancer treatment, it is timely to fingerprint the impact of olaparib treatment in cellular metabolism. Here, we report the biomolecular response of breast cancer cell lines with DNA damage repair defects to olaparib exposure. Following evaluation of olaparib sensitivity in breast cancer cell lines, we immunoprobed DNA double strand break foci and evaluated changes in cellular metabolism at various olaparib treatment doses using untargeted mass spectrometry-based metabolomics analysis. Following identification of altered features, we perfo

SUBMITTER: Berardi D 

PROVIDER: S-EPMC9367310 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature