A Prospective Validation Study of Lung Cancer Gene Panel Testing Using Cytological Specimens.

ABSTRACT: Background: Genetic panel tests require sufficient tissue samples, and therefore, cannot always be performed. Although collecting cytological specimens is easier than tissue collection, there are no validation studies on the diagnostic accuracy of lung cancer gene panel tests using cytology samples. Methods: Using an amplicon-based high-sensitivity next-generation sequencing panel test capable of measuring eight druggable genes, we prospectively enrolled consecutive patients who underwent diagnostic procedures. We evaluated the analysis accuracy rate, nucleic acid yield, and the quality of cytological specimens under brushing, needle aspiration, and pleural effusion. We then compared these specimens with collected tissue samples. Results: In 163 pr

SUBMITTER: Morikawa K 

PROVIDER: S-EPMC9367394 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature