A Novel In Vivo Model for Multiplexed Analysis of Callosal Connections upon Cortical Damage.

ABSTRACT: Brain damage is the major cause of permanent disability and it is particularly relevant in the elderly. While most studies focused on the immediate phase of neuronal loss upon injury, much less is known about the process of axonal regeneration after damage. The development of new refined preclinical models to investigate neuronal regeneration and the recovery of brain tissue upon injury is a major unmet challenge. Here, we present a novel experimental paradigm in mice that entails the (i) tracing of cortico-callosal connections, (ii) a mechanical lesion of the motor cortex, (iii) the stereological and histological analysis of the damaged tissue, and (iv) the functional characterization of motor deficits. By combining conventional microscopy with semi-automated 3D reconstruction, this appro

SUBMITTER: Gonzalez-Manteiga A 

PROVIDER: S-EPMC9368090 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature