Regulation of APD and Force by the Na⁺/Ca²⁺ Exchanger in Human-Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue.

ABSTRACT: The physiological importance of NCX in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is not well characterized but may depend on the relative strength of the current, compared to adult cardiomyocytes, and on the exact spatial arrangement of proteins involved in Ca²⁺ extrusion. Here, we determined NCX currents and its contribution to action potential and force in hiPSC-CMs cultured in engineered heart tissue (EHT). The results were compared with data from rat and human left ventricular tissue. The NCX currents in hiPSC-CMs were larger than in ventricular cardiomyocytes isolated from human left ventricles (1.3 ± 0.2 pA/pF and 3.2 ± 0.2 pA/pF for human ventricle and EHT, respectively, p < 0.05). SEA0400 (10 µM) markedly shortened the APD₉₀

SUBMITTER: Ismaili D 

PROVIDER: S-EPMC9368200 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature