Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis.

ABSTRACT:

Background

Pyroptosis is a catabolic process relevant to periodontal disorders for which interleukin-1β (IL-1β) inflammation is central to the pathophysiology of the disease. Despite platelet-rich fibrin (PRF) anti-inflammatory properties and its application to support periodontal regeneration, the capacity of PRF to modulate pyroptosis, specifically the production and release of IL-1β, remains unknown. The question arises whether PRF could regulate IL-1β release from macrophages in vitro.

Methods

To answer this question, RAW 264.7 macrophages and primary macrophages obtained from murine bone marrow were primed with PRF before being challenged by lipopolysaccharide (LPS). Cells were then analysed for the pyroptosis signalling components by gene expression analyses and IL-1β

SUBMITTER: Sordi MB 

PROVIDER: S-EPMC9368224 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature