Spectral Library-Based Single-Cell Proteomics Resolves Cellular Heterogeneity.

ABSTRACT: Dissecting the proteome of cell types and states at single-cell resolution, while being highly challenging, has significant implications in basic science and biomedicine. Mass spectrometry (MS)-based single-cell proteomics represents an emerging technology for system-wide, unbiased profiling of proteins in single cells. However, significant challenges remain in analyzing an extremely small amount of proteins collected from a single cell, as a proteome-wide amplification of proteins is not currently feasible. Here, we report an integrated spectral library-based single-cell proteomics (SLB-SCP) platform that is ultrasensitive and well suited for a large-scale analysis. To overcome the low MS/MS signal intensity intrinsically associated with a single-cell analysis, this approach takes an alte

SUBMITTER: Senavirathna L 

PROVIDER: S-EPMC9368228 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature