Visual Detection of Chicken Adulteration Based on a Lateral Flow Strip-PCR Strategy.

ABSTRACT: The aim of this study was to develop an accurate, easy-to-use, and cost-effective method for the detection of chicken adulteration based on polymerase chain reaction (PCR) and lateral flow strip (LFS). We compared six DNA extraction methods, namely the cetyltrimethylammonium bromide (CTAB) method, salt method, urea method, SDS method, guanidine isothiocyanate method, and commercial kit method. The chicken cytb gene was used as a target to design specific primers. The specificity and sensitivity of the PCR-LFS system were tested using a self-assembled lateral flow measurement sensor. The results showed that the DNA concentration obtained by salt methods is up to 533 ± 84 ng µL^-1, is a suitable replacement for commercial kits. The PCR-LFS method exhibits high specificity at an ann

SUBMITTER: Xu H 

PROVIDER: S-EPMC9368418 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature