Genetic analysis of the mobilization and leading regions of the IncN plasmids pKM101 and pCU1.

ABSTRACT: The conjugative IncN plasmids pKM101 and pCU1 have previously been shown to contain identical oriT sequences as well as conserved restriction endonuclease cleavage patterns within their tra regions. Complementation analysis and sequence data presented here indicate that these two plasmids encode essentially identical conjugal DNA-processing proteins. This region contains three genes, traI, traJ, and traK, transcribed in the same orientation from a promoter that probably lies within or near the conjugal transfer origin (oriT). Three corresponding proteins were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and complementation analysis confirmed that this region contains three tra complementation groups. All three proteins resemble proteins of the IncW plasmid R388

SUBMITTER: Paterson ES 

PROVIDER: S-EPMC93686 | biostudies-literature | 1999 Apr

REPOSITORIES: biostudies-literature