Six macrophage-associated genes in synovium constitute a novel diagnostic signature for osteoarthritis.

ABSTRACT:

Background

Synovial macrophages play important roles in the formation and progression of osteoarthritis (OA). This study aimed to explore the biological and clinical significance of macrophage-associated genes (MAGs) in OA.

Methods

The OA synovial gene expression profiles GSE89408 and GSE82107 were obtained from the GEO database. Single-sample gene set enrichment analysis (ssGSEA) and GSEA were employed to decipher differences in immune infiltration and macrophage-associated biological pathways, respectively. Protein-protein interaction (PPI) network analysis and machine learning were utilized to establish a macrophage-associated gene diagnostic signature (MAGDS). RT-qPCR was performed to test the expression of key MAGs in murine models.

Results

OA synovium presented

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9368762 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature