Unknown

Dataset Information

0

Bombyx mori C-Type Lectin (BmIML-2) Inhibits the Proliferation of B. mori Nucleopolyhedrovirus (BmNPV) through Involvement in Apoptosis.


ABSTRACT: C-type lectins (CTLs) are widely distributed in mammals, insects, and plants, which act as pattern recognition receptors (PRRs) to recognize pathogens and initiate immune responses. In this study, we identified a C-type lectin gene called BmIML-2 from the silkworm Bombyx mori. Its open reading frame (ORF) encodes 314 amino acids, which contain dual tandem C-type lectin-like domain (CTLD). BmIML-2 is highly expressed in the fat body and is significantly induced at 24 h after BmNPV infection. Moreover, overexpression of BmIML-2 dramatically inhibited the proliferation of BmNPV, and knockdown assay via siRNA further validated the inhibition of BmIML-2 on viral proliferation. In addition, transcript level detection of apoptosis-related genes and observation of apoptosis bodies implied that overexpression of BmIML-2 promoted BmNPV-induced apoptosis. Immunofluorescence analysis indicated that BmIML-2 distributed throughout the cytoplasm and was slightly concentrated in the cell membrane. Taken together, our results suggest that BmIML-2 could inhibit in the proliferation of BmNPV by facilitating cell apoptosis.

SUBMITTER: Mei X 

PROVIDER: S-EPMC9369074 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

<i>Bombyx mori</i> C-Type Lectin (BmIML-2) Inhibits the Proliferation of <i>B. mori</i> Nucleopolyhedrovirus (BmNPV) through Involvement in Apoptosis.

Mei Xianghan X   Li Chun C   Peng Peilin P   Wang Jue J   He Enxi E   Qiu Zhiyong Z   Xia Dingguo D   Zhao Qiaoling Q   Shen Dongxu D  

International journal of molecular sciences 20220728 15


C-type lectins (CTLs) are widely distributed in mammals, insects, and plants, which act as pattern recognition receptors (PRRs) to recognize pathogens and initiate immune responses. In this study, we identified a C-type lectin gene called <i>BmIML-2</i> from the silkworm <i>Bombyx mori</i>. Its open reading frame (ORF) encodes 314 amino acids, which contain dual tandem C-type lectin-like domain (CTLD). <i>BmIML-2</i> is highly expressed in the fat body and is significantly induced at 24 h after  ...[more]

Similar Datasets

| S-EPMC8396850 | biostudies-literature
| S-EPMC8708760 | biostudies-literature
| S-EPMC10299522 | biostudies-literature
| S-EPMC10779178 | biostudies-literature
2024-07-31 | GSE272804 | GEO
| S-EPMC5562830 | biostudies-literature
| S-EPMC6521139 | biostudies-literature
| S-EPMC9186105 | biostudies-literature
| S-EPMC8152266 | biostudies-literature
| S-EPMC4264868 | biostudies-literature