Regulatory T Cell Depletion Using a CRISPR Fc-Optimized CD25 Antibody.

ABSTRACT: Regulatory T cells (T_regs) are major drivers behind immunosuppressive mechanisms and present a major hurdle for cancer therapy. T_regs are characterized by a high expression of CD25, which is a potentially valuable target for T_reg depletion to alleviate immune suppression. The preclinical anti-CD25 (αCD25) antibody, clone PC-61, has met with modest anti-tumor activity due to its capacity to clear T_regs from the circulation and lymph nodes, but not those that reside in the tumor. The optimization of the Fc domain of this antibody clone has been shown to enhance the intratumoral T_reg depletion capacity. Here, we generated a stable cell line that produced optimized recombinant T_reg-depleting antibodies. A genome engineering stra

SUBMITTER: van Elsas MJ 

PROVIDER: S-EPMC9369266 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature