DNA Oxidative Damage as a Sensitive Genetic Endpoint to Detect the Genotoxicity Induced by Titanium Dioxide Nanoparticles.

ABSTRACT: The genotoxicity of nanomaterials has attracted great attention in recent years. As a possible occupational carcinogen, the genotoxic effects and underlying mechanisms of titanium dioxide nanoparticles (TiO₂ NPs) have been of particular concern. In this study, the effect of TiO₂ NPs (0, 25, 50 and 100 µg/mL) on DNA damage and the role of oxidative stress were investigated using human bronchial epithelial cells (BEAS-2B) as an in vitro model. After detailed characterization, the cytotoxicity of TiO₂ NPs was detected. Through transmission electron microscopy (TEM), we found that TiO₂ NPs entered the cytoplasm but did not penetrate deep into the nucleus of cells. The intracellular levels of reactive oxygen species (ROS) significantly increased in a

SUBMITTER: Chen Z 

PROVIDER: S-EPMC9370504 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature