Development of a simple and versatile in vitro method for production, stimulation, and analysis of bioengineered muscle.

ABSTRACT: In recent years, 3D in vitro modeling of human skeletal muscle has emerged as a subject of increasing interest, due to its applicability in basic studies or screening platforms. These models strive to recapitulate key features of muscle architecture and function, such as cell alignment, maturation, and contractility in response to different stimuli. To this end, it is required to culture cells in biomimetic hydrogels suspended between two anchors. Currently available protocols are often complex to produce, have a high rate of breakage, or are not adapted to imaging and stimulation. Therefore, we sought to develop a simplified and reliable protocol, which still enabled versatility in the study of muscle function. In our method, we have used human immortalized myoblasts cultured in a hydroge

SUBMITTER: Wells-Cembrano K 

PROVIDER: S-EPMC9371355 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature