Modification to axial tracking for mobile magnetic microspheres.

ABSTRACT: Three-dimensional particle tracking is a routine experimental procedure for various biophysical applications including magnetic tweezers. A common method for tracking the axial position of particles involves the analysis of diffraction rings whose pattern depends sensitively on the axial position of the bead relative to the focal plane. To infer the axial position, the observed rings are compared with reference images of a bead at known axial positions. Often the precision or accuracy of these algorithms is measured on immobilized beads over a limited axial range, while many experiments are performed using freely mobile beads. This inconsistency raises the possibility of incorrect estimates of experimental uncertainty. By manipulating magnetic beads in a bidirectional magnetic tweezer setu

SUBMITTER: Carlucci LA 

PROVIDER: S-EPMC9371438 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature