Effects of a gestational level of estradiol on cellular transition, migration, and inflammation in cervical epithelial and stromal cells.

ABSTRACT:

Problem

Estrogen (E2) is one of the main steroid hormones associated with pregnancy and parturition. High levels of E2 increase uterine contractions, promote fetal membrane weakening, and induce degradation of the cervical extracellular matrix (ECM). Current evidence supports the role of E2 in epithelial-to-mesenchymal transition (EMT) and inflammation in different cell types; however, its effects on the cellular components of the cervix are still unknown.

Method of study

In this study, we assessed the effects of gestational levels of E2 in: (a) the cellular transition of endocervical epithelial cells (EEC) and cervical stromal cells (CSC) in vitro using immunocytochemical staining and Western blot analyses for EMT markers (cytokeratin-18, E-cadherin, N-cadherin, SNAIL, and

SUBMITTER: Tantengco OAG 

PROVIDER: S-EPMC9371442 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature