Radius measurement via super-resolution microscopy enables the development of a variable radii proximity labeling platform.

ABSTRACT: The elucidation of protein interaction networks is critical to understanding fundamental biology as well as developing new therapeutics. Proximity labeling platforms (PLPs) are state-of-the-art technologies that enable the discovery and delineation of biomolecular networks through the identification of protein-protein interactions. These platforms work via catalytic generation of reactive probes at a biological region of interest; these probes then diffuse through solution and covalently "tag" proximal biomolecules. The physical distance that the probes diffuse determines the effective labeling radius of the PLP and is a critical parameter that influences the scale and resolution of interactome mapping. As such, by expanding the degrees of labeling resolution offered by PLPs, it is possibl

SUBMITTER: Oakley JV 

PROVIDER: S-EPMC9371666 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature