Hyper-swivel head domain motions are required for complete mRNA-tRNA translocation and ribosome resetting.

ABSTRACT: Translocation of messenger RNA (mRNA) and transfer RNA (tRNA) substrates through the ribosome during protein synthesis, an exemplar of directional molecular movement in biology, entails a complex interplay of conformational, compositional, and chemical changes. The molecular determinants of early translocation steps have been investigated rigorously. However, the elements enabling the ribosome to complete translocation and reset for subsequent protein synthesis reactions remain poorly understood. Here, we have combined molecular simulations with single-molecule fluorescence resonance energy transfer imaging to gain insights into the rate-limiting events of the translocation mechanism. We find that diffusive motions of the ribosomal small subunit head domain to hyper-swivelled positions, go

SUBMITTER: Nishima W 

PROVIDER: S-EPMC9371933 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature