Dataset Information

Culturable Endophytes Associated with Soybean Seeds and Their Potential for Suppressing Seed-Borne Pathogens.

ABSTRACT: Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. It has been well established during the last few decades that plant seeds harbor diverse microbes, some of which are vertically transmitted and important for plant health and productivity. In this study, we isolated culturable endophytic bacteria and fungi from soybean seeds and evaluated their antagonistic activities against common bacterial and fungal seed-borne pathogens of soybean. A total of 87 bacterial isolates and 66 fungal isolates were obtained. Sequencing of 16S rDNA and internal transcribed spacer amplicon showed that these isolates correspond to 30 and 15 different species of bacteria and fungi, respectively. Our antibacterial and antifungal activity assay showed that four fungal species and nine bacterial species have the potential to suppress the growth of at least one seed-borne pathogen tested in the study. Among them, Pseudomonas koreensis appears to have strong antagonistic activities across all the pathogens. Our collection of soybean seed endophytes would be a valuable resource not only for studying biology and ecology of seed endophytes but also for practical deployment of seed endophytes toward crop protection.

SUBMITTER: Kim J

PROVIDER: S-EPMC9372111 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Culturable Endophytes Associated with Soybean Seeds and Their Potential for Suppressing Seed-Borne Pathogens.

Kim Jiwon J Roy Mehwish M Ahn Sung-Ho SH Shanmugam Gnanendra G Yang Ji Sun JS Jung Ho Won HW Jeon Junhyun J

The plant pathology journal 20220801 4

Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. It has been well established during the last few decades that plant seeds harbor diverse microbes, some of which are vertically transmitted and im ...[more]

PMID: 35953051

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Project description:: Seed-borne fungi in 69 sunflower cultivars were evaluated which comprised 52 confectionery and 17 oilseed types. Seed coats were placed on both NP-10 (Nonylphenol Ethoxylate based surfacant -10) and potato dextrose agar (PDA) media to culture fungi. The rate of contamination among the different varieties was calculated by counting seed coats with fungal colonies. The rate of contamination in the confectionary group (88%) was significantly (p ≤ 0.05) higher than in the oilseed group (71%). Of the 52 confectionery varieties, the dominant fungi recovered were Verticilliumdahliae along with Alternaria spp., Fusarium spp., and Rhizopus spp., whereas the oilseed type varieties were contaminated with only V. dahliae. Molecular identification of fungal species via BLAST (Basic Alignment Search Tool) was performed on fungal sequences obtained from PCR (Polymerase Chain Reaction) analysis. The results included five Alternaria spp. that included Alternariatenuissima,Alternaria alternata,Alternaria helianthiinficiens, Alternaria longipes, and Alternaria tamaricis, three Fusarium spp. such as Fusariumoxysporum,Fusariumincarnatum, and Fusariumproliferatum, and V.dahliae and Cladosporiumcladosporioides. These were identified from pure fungal cultures recovered from seed coats. To efficiently control seed-borne fungi, four broad spectrum fungicides (carbendazim, triadimefon, caprio F-500, and flusilazole) were screened against V. dahliae isolate Gn3, which was isolated from a diseased LD 5009 sunflower plant. Flusilazole was selected based on its low half-maximal effective concentration value (EC50), 78.7 µg/mL. Seeds of diseased LD 5009 plants obtained from two different locations treated with formulated flusilazole fungicide at optimum parameters showed a significant (p ≤ 0.05) increase in seed germination and a decrease in contamination rate from 98% to less than 10%. The results affirmed that confectionery cultivars are much more susceptible to fungal contamination than oilseeds, and also that seed pretreatment is a suitable way to prevent the spread of soil- and seed-borne fungi in sunflower production.

Dataset Information

Culturable Endophytes Associated with Soybean Seeds and Their Potential for Suppressing Seed-Borne Pathogens.

Publications

Culturable Endophytes Associated with Soybean Seeds and Their Potential for Suppressing Seed-Borne Pathogens.

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