Inhibitory effects of LOXL2 knockdown on cellular functions of liver cancer stem cells.

ABSTRACT:

Background

Lysyl oxidase-like 2 (LOXL2) plays a role in tumor microenvironment formation and metastasis of hepatocellular carcinoma (HCC), which has a high mortality burden. Liver cancer stem cells (LCSCs) are related with the major malignant phenotypes of HCC. The function of LOXL2 in regulation of LCSCs remains unknown.

Methods

CD133⁺HepG2 and CD133⁺Hep3B cells were sorted by fluorescence-activated cell sorting (FACS) from two human hepatoblastoma cell lines. Spheroid formation, apoptosis, cell cycle, as well as transwell assays were performed upon LOXL2 knockdown in CD133⁺HepG2 and CD133⁺Hep3B cells. Protein and mRNA levels were quantified by Western blotting, immunofluorescence and reverse transcription-PCR (RT-PCR).

SUBMITTER: Li N 

PROVIDER: S-EPMC9372246 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature