The molecular diversity of transcriptional factor TfoX is a determinant in natural transformation in Glaesserella parasuis.

ABSTRACT: Natural transformation is a mechanism by which a particular bacterial species takes up foreign DNA and integrates it into its genome. The swine pathogen Glaesserella parasuis (G. parasuis) is a naturally transformable bacterium. The regulation of competence, however, is not fully understood. In this study, the natural transformability of 99 strains was investigated. Only 44% of the strains were transformable under laboratory conditions. Through a high-resolution melting curve and phylogenetic analysis, we found that genetic differences in the core regulator of natural transformation, the tfoX gene, leads to two distinct natural transformation phenotypes. In the absence of the tfoX gene, the highly transformable strain SC1401 lost its natural transformability. In

SUBMITTER: Tang X 

PROVIDER: S-EPMC9372613 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature