Development of a human antibody that exhibits antagonistic activity toward CC chemokine receptor 7.

ABSTRACT:

Background

CC chemokine receptor 7 (CCR7) is a member of G-protein-coupled receptor family and mediates chemotactic migration of immune cells and different cancer cells induced via chemokine (C-C motif) ligand 19 (CCL19) or chemokine (C-C motif) ligand 21 (CCL21). Hence, the identification of blockade antibodies against CCR7 could lead to the development of therapeutics targeting metastatic cancer.

Methods

CCR7 was purified and stabilized in its active conformation, and antibodies specific to purified CCR7 were screened from the synthetic M13 phage library displaying humanized scFvs. The in vitro characterization of selected scFvs identified two scFvs that exhibited CCL19-competitive binding to CCR7. IgG₄'s harboring selected scFv sequences were characteriz

SUBMITTER: Jang MS 

PROVIDER: S-EPMC9372883 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature