Current methodologies in protein ubiquitination characterization: from ubiquitinated protein to ubiquitin chain architecture.

ABSTRACT: Ubiquitination is a versatile post-translational modification (PTM), which regulates diverse fundamental features of protein substrates, including stability, activity, and localization. Unsurprisingly, dysregulation of the complex interaction between ubiquitination and deubiquitination leads to many pathologies, such as cancer and neurodegenerative diseases. The versatility of ubiquitination is a result of the complexity of ubiquitin (Ub) conjugates, ranging from a single Ub monomer to Ub polymers with different length and linkage types. To further understand the molecular mechanism of ubiquitination signaling, innovative strategies are needed to characterize the ubiquitination sites, the linkage type, and the length of Ub chain. With advances in chemical biology tools, computational metho

SUBMITTER: Sun M 

PROVIDER: S-EPMC9373315 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature