Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima.

ABSTRACT:

Background

Apple production in Sweden and elsewhere is being threatened by the fungus, Neonectria ditissima, which causes a disease known as European canker. The disease can cause extensive damage and the removal of diseased wood and heavily infected trees can be laborious and expensive. Currently, there is no way to eradicate the fungus from infected trees and our knowledge of the infection process is limited. Thus, to target and modify genes efficiently, the genetic transformation technique developed for N. ditissima back in 2003 was modified.

Results

The original protocol from 2003 was upgraded to use enzymes currently available in the market for making protoplasts. The protoplasts were viable, able to uptake foreign DNA, and able to regenerate back into a mycelial colony

SUBMITTER: Velez H 

PROVIDER: S-EPMC9373326 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature