Estimation of the impact of three different bioinformatic pipelines on sheep nemabiome analysis.

ABSTRACT:

Background

Next-generation sequencing (NGS) has provided an alternative strategy to study the composition of nematode communities with increased resolution and sensitivity. However, the handling and processing of gigabytes worth of amplicon sequence data produced by an NGS platform is still a major hurdle, limiting the use and adoption of faster and more convenient analysis software.

Methods

In total 32 paired, fecal samples from Swedish sheep flocks were cultured and the larvae subsequently harvested subjected to internal transcribed spacer 2 (ITS2) amplicon sequencing using the PacBio platform. Samples were analyzed with three different bioinformatic pipelines, i.e. the DADA2, Mothur and SCATA pipelines, to determine species composition and richness.

Results

For the

SUBMITTER: Baltrusis P 

PROVIDER: S-EPMC9373329 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature