Different serotypes of Escherichia coli flagellin exert identical adjuvant effects.

ABSTRACT: Bacterial flagellin is a potent powerful adjuvant, which exerts its adjuvant activity by activating the Toll-like receptor 5 (TLR5) signaling pathway to induce host pro-inflammatory responses. Flagellin of Salmonella typhimurium (S. typhimurium) has shown strong adjuvant effects for a variety of vaccine candidates, however, the adjuvanticity of different serotypes of Escherichia coli (E. coli) flagellin (FliC) is unclear. To explore the adjuvant activity of different serotypes of E. coli flagellin, FliC_H1, FliC_H7, and FliC_H19 recombinant flagellins were prokaryotically-expressed and purified. The adjuvanticity of three recombinant flagellins was evaluated by analyzing their abilities to induce the IL-8 production in human colorectal adenocarcinoma (Caco-2)

SUBMITTER: Pang S 

PROVIDER: S-EPMC9373361 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature