Comprehensive analysis of RNA m6A methylation in pressure overload-induced cardiac hypertrophy.

ABSTRACT:

Aim

To analyze and compare the mRNA N⁶-methyladenosine modifications in transverse aortic constriction induced mice hearts and normal mice hearts.

Materials and methods

Colorimetric quantification was used to probe the changes in m⁶A modifications in the total RNA. The expression of m⁶A-related enzymes was analyzed via qRT-PCR and western blotting. RNA-seq and MeRIP-seq were performed to identify genes with differences in m⁶A modifications or expression in the transcriptome profile.

Results

Compared with the control group, the TAC group exhibited higher m⁶A methylation levels. FTO and WTAP were downregulated after TAC, while METTL3 was significantly downregulated at the protein level. MeRIP-seq revealed that 1179

SUBMITTER: Li W 

PROVIDER: S-EPMC9373449 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature