Study of Calcium Signaling in Astrocytes with a Novel Endoplasmic Reticulum-Targeted GCaMP Sensor.

ABSTRACT: The endoplasmic reticulum (ER), the major organelle for the storage of Ca²⁺ , maintains a concentration of Ca²⁺ much higher than in the cytosol or other subcellular organelles, such as the mitochondria. A variety of tools have been developed for measuring Ca²⁺ activity in neuronal and glial cells, but most of these sensors target either the plasma membrane (PM) or the cytosol. Though these sensors are important for measuring Ca²⁺ transients, they lack the capability to measure activity in the periphery of the ER or to measure low-amplitude events resulting from Ca²⁺ exchange between the ER and other organelles, such as the mitochondria. We recently developed an ER-targeted GCaMP6f anchored to the cytosolic side of the ER that can measu

SUBMITTER: Aryal SP 

PROVIDER: S-EPMC9373517 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature