RNA N⁶ -methyladenosine reader YTHDC1 is essential for TGF-beta-mediated metastasis of triple negative breast cancer.

ABSTRACT: RNA N⁶ -methyladenosine (m⁶A) modification and its regulators fine tune gene expression and contribute to tumorigenesis. This study aims to uncover the essential role and the underlying molecular mechanism(s) of the m⁶A reader YTHDC1 in promoting triple negative breast cancer (TNBC) metastasis.

Methods

In vitro and in vivo models were employed to determine the pathological function of YTHDC1 in TNBC metastasis. To identify bona fide YTHDC1 target RNAs, we conducted RNA-seq, m⁶A-seq, and RIP-seq, followed by integrative data analysis and validation assays.

Results

By analyzing The Cancer Genome Atlas (TCGA) dataset, we found that elevated expression of YTHDC1 is positively correlated with poo

SUBMITTER: Tan B 

PROVIDER: S-EPMC9373808 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature