Lgr5⁺ cells are required and dynamically participate in olfactory epithelium regeneration: a revisiting shows Lgr5 expression in multiple cell lineages.

ABSTRACT: Olfactory sensory neurons (OSNs) located in the olfactory epithelium (OE) detect thousands of volatile environmental odors to form the sense of smell. OSNs are generated from basal cells, which show the characteristics of progenitor/stem cells. In the mammalian OE, persistent neurogenesis occurs during lifetime, providing a unique model to study the tissue turnover and fate determination of stem cells. Methods: Immunohistochemical analysis and RNAscope in situ hybridization indicated the localization of leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) in the intact and injured OE. Lineage tracing was conducted to analyze the dynamic role of Lgr5⁺ cells in the OE homeostasis and regeneration. We also used DTR-driven genetic depletion of Lgr5⁺ c

SUBMITTER: Ren W 

PROVIDER: S-EPMC9373817 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature